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Living Modified Organism
(LMO)
The image below identifies the LMO through its unique identifier, trade name and a link to this page of the BCH. Click on it to download a larger image on your computer. For help on how to use it go to the LMO quick-links page.
Insect-resistant and herbicide-tolerant maize
EN
BT11 × NK603 × TC1507
Yes
SYN-BTØ11-1 × DAS-Ø15Ø7-1 × MON-ØØ6Ø3-6
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Organization:Syngenta Seeds GmbH ()Private sector (business and industry)Syngenta Seeds GmbH Zum Knipkenbach 20Bad Salzuflen,
32107, GermanyPhone: +49 52 22 5308-0,Fax: +49 52 22 5308-12,Email: info.seeds@syngenta.com,Website: http://www.syngenta-seeds.de/de/,
The maize (Zea mays) was produced through cross‑breeding of modified parental varieties SYN-BTØ11-1, MON-ØØ6Ø3-6 and × DAS-Ø15Ø7-1.
Maize SYN-BTØ11-1 was modified for resistance to the Lepidoptera insect European corn borer (Ostrinia nubilalis) and tolerance to glufosinate-ammonium herbicides. To achieve resistance to the European corn borer, the maize expresses cry1Ab gene from Bacillus thuringiensis subsp. kurstaki, which have a pore forming mode-of-action in the epithelial lining of feeding larvae; cry1Ab produces a crystal delta-endotoxin that binds to specific midgut receptors in the insect larva, forming pores that disrupt osmotic balance and cause fatal cell lysis. To achieve resistance to glufosinate, the maize expresses the gene from Streptomyces viridochromogenes encoding phosphinothricin N-acetyltransferase encoding gene, which inactivates the active compound L-phosphinothricin through acetylation of the primary amino group.
Maize SYN-BTØ11-1 was modified for resistance to the Lepidoptera insect European corn borer (Ostrinia nubilalis) and tolerance to glufosinate-ammonium herbicides. To achieve resistance to the European corn borer, the maize expresses cry1Ab gene from Bacillus thuringiensis subsp. kurstaki, which have a pore forming mode-of-action in the epithelial lining of feeding larvae; cry1Ab produces a crystal delta-endotoxin that binds to specific midgut receptors in the insect larva, forming pores that disrupt osmotic balance and cause fatal cell lysis. To achieve resistance to glufosinate, the maize expresses the gene from Streptomyces viridochromogenes encoding phosphinothricin N-acetyltransferase encoding gene, which inactivates the active compound L-phosphinothricin through acetylation of the primary amino group.
Maize DAS-Ø15Ø7-1 was modified for resistance to the lepidopteran European corn borer (Ostrinia nubilalis) and tolerance to herbicide glufosinate ammonium. To achieve lepidopteran resistance, the modified corn Bacillus thuringiensis expresses the Cry1F protein (delta-endotoxin/crystal protein),which has a pore forming mode of action in the epithelial lines of the feeding insect larvae. To achieve tolerance to the herbicide glufinosinate, the modified corn expresses Streptomyces viridochromogenes phosphinothricin N-acetyltransferase, which acetylates the glufosinate ammonium herbicide to form a non-toxic compound, preventing the inhibition of glutamine synthetase and thus preserving ammonia levels and nitrogen metabolism in the plant.
Maize MON-ØØ6Ø3-6 was modified for tolerance to glyphosate herbicides. To achieve glyphosate tolerance, the maize expresses enzyme 5-enolpyruvylshikimate-3-phosphate synthase, encoded by the cp4 epsps gene from Agrobacterium tumefaciens strain CP4, which has reduced binding affinity for glyphosate and allows continued synthesis of aromatic amino acids through the shikimate pathway in the presence of the herbicide.
Maize MON-ØØ6Ø3-6 was modified for tolerance to glyphosate herbicides. To achieve glyphosate tolerance, the maize expresses enzyme 5-enolpyruvylshikimate-3-phosphate synthase, encoded by the cp4 epsps gene from Agrobacterium tumefaciens strain CP4, which has reduced binding affinity for glyphosate and allows continued synthesis of aromatic amino acids through the shikimate pathway in the presence of the herbicide.
The term “Recipient organism” refers to an organism (either already modified or non-modified) that was subjected to genetic modification, whereas “Parental organisms” refers to those that were involved in cross breeding or cell fusion.
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BCH-LMO-SCBD-14776-18 Living Modified Organism MON-ØØ6Ø3-6 - Roundup Ready™ maizeMonsanto | Resistance to herbicides (Glyphosate)
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BCH-LMO-SCBD-14797-16 Living Modified Organism SYN-BTØ11-1 - Agrisure™ CB/LLSyngenta | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), European corn borer (Ostrinia nubilalis)), Resistance to herbicides (Glufosinate)
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BCH-ORGA-SCBD-246-6 Organism Zea mays (Maize, Corn, MAIZE)Crops
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BCH-LMO-SCBD-14841-16 Living Modified Organism DAS-Ø15Ø7-1 - Herculex™ I maizeDow AgroSciences, Pioneer Hi-Bred International Inc. | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths), European corn borer (Ostrinia nubilalis)), Resistance to herbicides (Glufosinate)
EN
pZO1502 derived from pUC18, pNOV1300, PHI8999A derived from plasmid PHP8999, PV-ZMGT32
EN
- Cross breeding
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Some of these genetic elements may be present as fragments or truncated forms. Please see notes below, where applicable.
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BCH-GENE-SCBD-100287-7 CaMV 35S promoter | Cauliflower mosaic virus (CaMV)Promoter
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BCH-GENE-SCBD-103625-3 Alcohol dehydrogenase 1, intron 6 | Zea mays (Maize, Corn, MAIZE)Intron
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BCH-GENE-SCBD-14985-12 Cry1Ab | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-GENE-SCBD-100269-8 Nopaline Synthase Gene Terminator | Agrobacterium tumefaciens (Agrobacterium)Terminator
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BCH-GENE-SCBD-103867-2 Alcohol dehydrogenase 1, intron 2 | Zea mays (Maize, Corn, MAIZE)Intron
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BCH-GENE-SCBD-15002-5 Phosphinothricin N-acetyltransferase | Streptomyces viridochromogenes (STRVR)Protein coding sequence | Resistance to herbicides (Glufosinate)
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BCH-GENE-SCBD-100362-7 Ubiquitin gene promoter | Zea mays (Maize, Corn, MAIZE)Promoter
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BCH-GENE-SCBD-100887-5 Vegetative insecticidal protein 3Aa20 | Bacillus thuringiensis (Bt, Bacillus, BACTU)Protein coding sequence | Resistance to diseases and pests (Insects, Lepidoptera (butterflies and moths))
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BCH-GENE-SCBD-101406-4 Phosphoenolpyruvate carboxylase, intron 9 | Zea mays (Maize, Corn, MAIZE)Intron
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BCH-GENE-SCBD-15003-7 Phosphomannose Isomerase gene | Escherichia coli (ECOLX)Protein coding sequence | Mannose tolerance,Selectable marker genes and reporter genes
DNA insert from SYN-BTØ11-1 (vector pZO1502)
The DNA insert from maize SYN-BTØ11-1 contained two genetic cassettes expressing the cry1Ab gene from Bacillus thuringiensis subsp. kurstaki and the phosphinothricin N-acetyltransferase (PAT) encoding gene from Streptomyces viridochromogenes. Both cassettes were introduced by particle acceleration (biolistic) transformation.
(1) The cry1Ab gene is under the control of the 35S Cauliflower mosaic virus promoter and the Agrobacterium tumefaciens nopaline synthase gene terminator. An alcohol dehydrogenase 1 intron (intron 6) from Zea mays was included to enhance expression of the cry1Ab sequence from Bacillus thuringiensis. High levels of transcription are expected due to the strong constitutive promoter accompanied by intron-mediated enhancement.
(2) The pat gene is regulated by the 35S promoter from Cauliflower mosaic virus and the nopaline synthase gene terminator from Agrobacterium tumefaciens. The Zea mays alcohol dehydrogenase 1 intron (intron 2) from was also included to enhance expression of the phosphinothricin N-acetyltransferase sequence from Streptomyces viridochromogenes. High levels of transcription are expected due to the strong constitutive promoter accompanied by intron-mediated enhancement.
Note
The DNA insert from maize SYN-BTØ11-1 contained two genetic cassettes expressing the cry1Ab gene from Bacillus thuringiensis subsp. kurstaki and the phosphinothricin N-acetyltransferase (PAT) encoding gene from Streptomyces viridochromogenes. Both cassettes were introduced by particle acceleration (biolistic) transformation.
(1) The cry1Ab gene is under the control of the 35S Cauliflower mosaic virus promoter and the Agrobacterium tumefaciens nopaline synthase gene terminator. An alcohol dehydrogenase 1 intron (intron 6) from Zea mays was included to enhance expression of the cry1Ab sequence from Bacillus thuringiensis. High levels of transcription are expected due to the strong constitutive promoter accompanied by intron-mediated enhancement.
(2) The pat gene is regulated by the 35S promoter from Cauliflower mosaic virus and the nopaline synthase gene terminator from Agrobacterium tumefaciens. The Zea mays alcohol dehydrogenase 1 intron (intron 2) from was also included to enhance expression of the phosphinothricin N-acetyltransferase sequence from Streptomyces viridochromogenes. High levels of transcription are expected due to the strong constitutive promoter accompanied by intron-mediated enhancement.
Note
- The plasmid carrying the DNA transfer was digested by restriction enzyme NotI and only the fragments containing the two expression cassettes were inserted into the LM maize. The AMPr gene encoding ampicillin resistance and an origin of replication were not inserted.
- Southern blot analysis confirmed the absence of unwanted DNA fragments in the transformant generations.
DNA insert from DAS-Ø15Ø7-1 (vector PHI8999A)
The DNA insert from maize DAS-Ø15Ø7-1 contained two genetic cassettes expressing the cry1F gene from Bacillus thuringiensis and the phosphinothricin N-acetyltransferase (pat) gene from Streptomyces viridochromogenes.
(1) The cry1F gene regulated by the promoter and first exon and intron of the maize ubiquitin gene. The 3' terminator sequence used was the 3' polyadenylation signal from ORF25 (Agrobacterium tumefaciens) (see footnote*). Due to the constitutive nature of the promoter, high levels of expression in all plant tissues are expected from this cassette.
(2) The pat coding sequence was under control of the Cauliflower mosaic virus 35S promoter and terminator. Due to the viral nature of the promoter, high levels of transcriptional expression in all plant tissues are expected from this genetic cassette.
Note
- The coding sequence of both genes has been optimized to achieve a high level of expression in maize.
- The sequences of the complete cry1F and pat genes are identical to those in the original plasmid. The proteins produced in the modified plants are the ones intended, including a leucine residue (replacing a phenylalanine) at position 604 (of 605 amino acids in total) of Cry1F. This modification was introduced to create a specific restriction site for cloning purposes.
- Molecular analyses of the transformed plant show that the event TC1507 contains one site of integration of the introduced DNA which includes a full-length of the DNA fragment used for transformation (i.e. the ~6235 bp of DNA cassette containing the cry1F and pat genes) and an additional copy of the cry1F gene lacking the majority of the associated ubiquitin regulatory sequences.
- Southern analysis using a cry1F probe carried out by the European Food Safety Authority (EFSA; see document below) also revealed the presence of two cry1F inserts. The first represented the intact gene from the expression cassette. The second insert was a truncated cry1F fragment of 335bp, which is located at the 5’ end of the insertion locus. In addition, analysis of the sequences adjacent to the insert of fragment PHI8999A revealed DNA fragments that correspond to small segments from PHI8999A, including incomplete sequences from the pat coding sequence, the maize ubiquitin promoter and the terminator from Agrobacterium tumefaciens. Furthermore, different fragments of chloroplast DNA and a number of sequences with similarity to retrotransposons are also present in the border region of the insert.
Footnote *: The EFSA document indicates that the 3’ sequence from the A. tumefaciens mannopine synthase gene was used as terminator of the cry1F gene.
DNA insert from MON-ØØ6Ø3-6 (vector PV-ZMGT32)
The DNA insert from maize MON-ØØ6Ø3-6 contains two adjacent genetic cassettes expressing the 5-enolpyruvylshikimate-3-phosphate synthase gene (CP4 EPSPS) from Agrobacterium tumefaciens strain CP4. Both cassettes were introduced by particle acceleration (biolistic) transformation.
(1) The cp4 epsps gene is under the regulation of the rice actin promoter (P-Ract1) and the rice actin intron (I-Ract1) and the 3' UTR region of the nopaline synthase gene (nos 3'). An intron from Oryza sativa (I-Ract1) was included to enhance expression of the cp4 epsps sequence from Agrobacterium tumefaciens, and Arabidopsis thaliana chloroplast transit peptide 2 to direct the translated CP4 EPSPS protein to the chloroplast.
(2) The cp4 epsps gene is regulated by the enhanced Cauliflower mosaic virus 35S promoter (e35S) and the 3' UTR region of the nopaline synthase gene (nos 3'). An intron from the Zea mays heat shock protein 70 (HSP70) was included to enhance expression of the cp4 epsps sequence from Agrobacterium tumefaciens, and Arabidopsis thaliana chloroplast transit peptide 2 to direct the translated CP4 EPSPS protein to the chloroplast.
Note
- The plasmid carrying the DNA transfer was digested by restriction enzyme MluI and only the fragments containing the two expression cassettes were inserted into the LM maize. The nptII gene encoding neomycin phosphotransferase II (NPTII) for kanamycin resistance and an origin of replication were not inserted.
- Maize line NK603 contains one insertion site containing a single copy of the linear DNA of PV-ZMGT32 used for transformation. Both cp4 epsps gene cassettes within the single insert which are intact.
- Promoter e35S is a 0.61Kb long sequence containing the promoter and leader for the cauliflower mosaic virus (CaMV) 35S RNA containing the duplicated enhancer region.
For additional information on this LMO, please refer to the records of the parental LMOs.
EN
- Feed
- Food
- SYN-BTØ11-1 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) [ English ]
- Event-specific Method for the Quantification of Maize MIR162 Using Real-time PCR.pdf [ English ]
- DAS-Ø15Ø7-1 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- MON-ØØ6Ø3-6 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- SYN-BTØ11-1 - EU Reference Laboratory for GM Food and Feed (EURL-GMFF) ( JRC ) [ English ]
- SYN-BTØ11-1 - CropLife International Detection Methods Database ( CropLife ) [ English ]
- DAS-Ø15Ø7-1 - CropLife International Detection Methods Database ( CropLife ) [ English ]
- MON-ØØ6Ø3-6 - CropLife International Detection Methods Database ( CropLife ) [ English ]
EN
Pertaining to parental LMO MON-ØØ6Ø3-6, glyphosate specifically binds to and inactivates the enzyme EPSPS, which is part of an important plant biochemical pathway called the shikimate pathway. The shikimate pathway is involved in the biosynthesis of the aromatic amino acids tyrosine, phenylalanine and tryptophan, as well as other aromatic compounds. When conventional plants are treated with glyphosate they cannot produce the aromatic amino acids essential to their survival. The modified maize line permits farmers to use glyphosate-containing herbicides for weed control in the cultivation of maize. The EPSPS enzyme is present in all plants, bacteria and fungi, but not in animals, which do not synthesize their own aromatic amino acids. Thus, EPSPS is normally present in food derived from plant and microbial sources.
- EUginius: Bt11 x DAS1507 x NK603 [URL] [ English ]
- ISAAA GM Approval database: Bt11 x TC1507 x NK603 [URL] [ English ]
